VASP Antikörper (pSer239)

Produktnummer ABIN126912

Dieses Anti-VASP-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von VASP in WB, IF, IP, FACS und EIA. Geeignet für Human und Maus.

Kurzübersicht für VASP Antikörper (pSer239) (ABIN126912)

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VASP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Klon: 16C2

Produktdetails anti-VASP Antikörper

Bindungsspezifität: pSer239

Spezifität: AM00156PU-N recognizes VASP only, when Ser 239 is phosphorylated, a site preferred by cAMP-depedent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, mab AM00156PU-N (clone 16C2) is able to monitor the phosphorylation state of VASP serine 239 as well as PKA activity.

Produktmerkmale: Synonyms: Vasodilator-stimulated phosphoprotein

Aufreinigung: Purified from serum-free cell culture supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Immunogen: Synthetic phosphopeptide conjugated to KLHEpitope: Phosphoserine 239 RKVpSKQE

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA (0.05 μg/mL). Immunoblotting (0.5 μg/mL for HRPO/ECL detection). Recommended blocking buffer: BSA/Tween 20 based blocking and blot incubation buffer. Flow Cytometry. Immunoprecipitation (1-10 μg per 10^6 pervanadate-treated A431 cells). Immunocytochemistry (1-10 μg/mL, may tolerate 0.5 % formaldehyde fixation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 2x PBS containing 0.09 % Sodium Azide, PEG and Sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquot and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be storedat 2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles!

Haltbarkeit: 12 months

Details zu VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Andere Bezeichnung: VASP

Hintergrund: VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling. VASP is expressed in almost all human and animal cell lines, paricularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.Synonyms: Vasodilator-stimulated phosphoprotein

Gen-ID: 7408

UniProt: P50552

Pathways: T-Zell Rezeptor Signalweg, Regulation of Actin Filament Polymerization, Tube Formation