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VASP Antikörper (pSer157)

VASP Reaktivität: Human, Maus WB, IF, IP, FACS, EIA Wirt: Maus Monoclonal 5C6 unconjugated
Produktnummer ABIN126908
  • Target Alle VASP Antikörper anzeigen
    VASP (Vasodilator-Stimulated phosphoprotein (VASP))
    Bindungsspezifität
    • 18
    • 17
    • 15
    • 10
    • 9
    • 8
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer157
    Reaktivität
    • 137
    • 64
    • 58
    • 12
    • 11
    • 9
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Maus
    Wirt
    • 116
    • 20
    • 2
    Maus
    Klonalität
    • 111
    • 27
    Monoklonal
    Konjugat
    • 80
    • 10
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser VASP Antikörper ist unkonjugiert
    Applikation
    • 105
    • 51
    • 50
    • 27
    • 22
    • 17
    • 14
    • 14
    • 11
    • 11
    • 5
    • 4
    • 4
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)
    Spezifität
    AM00155PU-N recognizes VASP only, when Ser 157 is phosphorylated, a site preferred by cAMP-depedent protein kinase (PKA). The antibody does not cross-react with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, AM00155PU-N (clone 5C6) is able to monitor the phosphorylation state of VASP serine 157 as well as PKA activity.
    Produktmerkmale
    Synonyms: Vasodilator-stimulated phosphoprotein
    Aufreinigung
    Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
    Immunogen
    Synthetic phosphopeptide conjugated to KLHEpitope: Phosphoserine 157 RRVpSNAG
    Klon
    5C6
    Isotyp
    IgG1
    Top Product
    Discover our top product VASP Primärantikörper
  • Applikationshinweise
    Western blot ( 0.5 μg/mL for HRPO/ECL detection). Recommended: Casein/Tween 20 basedblocking and blot incubation buffer. ELISA (0.05 μg/mL). Immunoprecipitation (1 - 10 μg per 10^6 pervanadate-treated A431 cells). Immunocytochemistry (1 - 10 μg/mL, may tolerate 0.5 % formaldehyde fixation). Flow cytometry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Restore with 1 mL H2O (15 min, RT).
    Buffer
    PBS with 0.09 % Sodium Azide, PEG and Sucrose
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquot and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be storedat 2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles!
    Haltbarkeit
    12 months
  • Iyú, Glenn, White, Fox, Heptinstall: "Adenosine derived from ADP can contribute to inhibition of platelet aggregation in the presence of a P2Y12 antagonist." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, Issue 2, pp. 416-22, (2011) (PubMed).

  • Target
    VASP (Vasodilator-Stimulated phosphoprotein (VASP))
    Andere Bezeichnung
    VASP (VASP Produkte)
    Synonyme
    vasp antikoerper, MGC80889 antikoerper, wu:fb23b04 antikoerper, wu:fk84g05 antikoerper, zgc:110347 antikoerper, si:dkey-113g17.1 antikoerper, si:ch211-202c21.1 antikoerper, DDBDRAFT_0188463 antikoerper, DDBDRAFT_0229340 antikoerper, DDB_0188463 antikoerper, DDB_0229340 antikoerper, VASP antikoerper, vasodilator stimulated phosphoprotein S homeolog antikoerper, vasodilator stimulated phosphoprotein b antikoerper, protein enabled antikoerper, vasodilator-stimulated phosphoprotein antikoerper, vasodilator stimulated phosphoprotein a antikoerper, vasodilator stimulated phosphoprotein antikoerper, vasp.S antikoerper, vaspb antikoerper, LOC5573842 antikoerper, CpipJ_CPIJ004706 antikoerper, CpipJ_CPIJ004707 antikoerper, vasP antikoerper, vasp antikoerper, VASP antikoerper, vaspa antikoerper, Vasp antikoerper
    Hintergrund
    VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling. VASP is expressed in almost all human and animal cell lines, paricularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation. Molecular Weight: 46/50 kDaSynonyms: Vasodilator-stimulated phosphoprotein
    Gen-ID
    7408
    UniProt
    P50552
    Pathways
    T-Zell Rezeptor Signalweg, Regulation of Actin Filament Polymerization, Tube Formation
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