VASP Antikörper (pSer157) (FITC)

Produktnummer ABIN126907

Dieses Maus Monoklonal-Antikörper erkennt spezifisch VASP in IF und FACS. Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 1 Publikation erwähnt.

Kurzübersicht für VASP Antikörper (pSer157) (FITC) (ABIN126907)

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VASP Antikörper ist konjugiert mit FITC

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 5C6

Produktdetails anti-VASP Antikörper (FITC)

Bindungsspezifität: pSer157

Spezifität: This antibody recognizes VASP only, when Ser157 is phosphorylated, a site preferred by cAMP-dependent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, this antibody is able to monitor the phosphorylation state of VASP Serine157 as well as PKA activity.

Produktmerkmale: Synonyms: Vasodilator-stimulated phosphoprotein

Aufreinigung: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Immunogen: Phosphopeptide conjugated to KLH. Epitope: Phosphoserine 157AA Sequence: R-R-V-pS-N-A-G

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Immunocytochemistry: 1-10 μg/mL (may tolerate 0.5 % Formaldehyde fixation). For Immunoblotting and Immunoprecipitation use ABIN126908 and ABIN126905.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: 2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Aliquote and freeze (in liquid nitrogen at -20 °C to -80 °C). Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Referenzen für anti-VASP Antikörper (FITC) (ABIN126907)

Iyú, Glenn, White, Fox, Heptinstall: "Adenosine derived from ADP can contribute to inhibition of platelet aggregation in the presence of a P2Y12 antagonist." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, Issue 2, pp. 416-22, (2011) (PubMed).

Details zu VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Andere Bezeichnung: VASP

Hintergrund: VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines, particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.Synonyms: Vasodilator-stimulated phosphoprotein

Molekulargewicht: 46/50 kDa

Gen-ID: 7408

UniProt: P50552

Pathways: T-Zell Rezeptor Signalweg, Regulation of Actin Filament Polymerization, Tube Formation