VASP Antikörper (pSer157) (Biotin)

Produktnummer ABIN126905

Produktdetails anti-VASP Antikörper (Biotin)

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Bindungsspezifität: pSer157

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VASP Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Spezifität: This antibody recognizes VASP only, when Ser 157 is phosphorylated, a site preferred by cAMP-dependent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, antibody VASP-5C6 is able to monitor the phosphorylation state of VASP serine 157 as well as PKA activity.

Produktmerkmale: Synonyms: Vasodilator-stimulated phosphoprotein

Aufreinigung: Size exclusion chromatography

Immunogen: Synthetic phosphopeptide conjugated to KLH

Klon: 5C6

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 μg per 10^6 pervanadate-treated A431 cells. Immunocytochemistry: 1 - 10 μg/mL, Mab VASP-5C6 may tolerate 0.5 % formaldehydefixation. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS with 0.09 % Sodium Azide, PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody (Aliquote and freeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Referenzen für anti-VASP Antikörper (Biotin) (ABIN126905)

Iyú, Glenn, White, Fox, Heptinstall: "Adenosine derived from ADP can contribute to inhibition of platelet aggregation in the presence of a P2Y12 antagonist." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, Issue 2, pp. 416-22, (2011) (PubMed).

Details zu VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Andere Bezeichnung: VASP (VASP Produkte)

Synonyme: vasp antikoerper, MGC80889 antikoerper, wu:fb23b04 antikoerper, wu:fk84g05 antikoerper, zgc:110347 antikoerper, si:dkey-113g17.1 antikoerper, si:ch211-202c21.1 antikoerper, DDBDRAFT_0188463 antikoerper, DDBDRAFT_0229340 antikoerper, DDB_0188463 antikoerper, DDB_0229340 antikoerper, VASP antikoerper, vasodilator stimulated phosphoprotein S homeolog antikoerper, vasodilator stimulated phosphoprotein b antikoerper, protein enabled antikoerper, vasodilator-stimulated phosphoprotein antikoerper, vasodilator stimulated phosphoprotein a antikoerper, vasodilator stimulated phosphoprotein antikoerper, vasp.S antikoerper, vaspb antikoerper, LOC5573842 antikoerper, CpipJ_CPIJ004706 antikoerper, CpipJ_CPIJ004707 antikoerper, vasP antikoerper, vasp antikoerper, VASP antikoerper, vaspa antikoerper, Vasp antikoerper

Hintergrund: VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines, particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filamentSynonyms: Vasodilator-stimulated phosphoprotein

Gen-ID: 7408

UniProt: P50552

Pathways: T-Zell Rezeptor Signalweg, Regulation of Actin Filament Polymerization, Tube Formation