NCOA1 Antikörper (pTyr416) (Biotin)

Produktnummer ABIN126892

Dieses Maus Monoklonal-Antikörper erkennt spezifisch NCOA1 in WB, EIA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für NCOA1 Antikörper (pTyr416) (Biotin) (ABIN126892)

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NCOA1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 9A6

Produktdetails anti-NCOA1 Antikörper (Biotin)

Bindungsspezifität: pTyr416

Spezifität: This antibody specifically interacts with activated src phosphorylated at Tyr 416 and with src family kinases phosphorylated at the corresponding Tyr in the activation loop. The antibody does not crossreact with the non-phosphorylated form of src nor with unrelated phosphorylation sites.

Produktmerkmale: Synonyms: c-Src, pp60c-src, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src

Aufreinigung: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Immunogen: Synthetic phosphopeptide conjuagted to KLH

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Flow Cytometry. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL in PBS/0.09 % Na-Azide/PEG and Sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody (Aliquote and freeze in liquid nitrogen)at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu NCOA1

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Andere Bezeichnung: SRC1

Hintergrund: Src is the best understood member of a family of nine tyrosine kinases regulating cellular responses to extracellular stimuli. Src becomes activated upon dephosphorylation of Tyr 527, leading to a conformational change of the src molecule towards an open, active conformation which allows binding to other proteins via the SH2 and SH3 domain. Activated src phosphorylates a variety of substrates involved in the mitogenic signalling pathway, cytoskeletal organization, cell-substrate adhesion, cell-cell adhesion, cell-cell communication, and RNA processing.Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src

Gen-ID: 6714

UniProt: P12931

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha