Nitrotyrosine Antikörper

Produktnummer ABIN126845

Der Maus Monoklonal Anti-Nitrotyrosine-Antikörper wurde für WB und EIA validiert. Er ist geeignet, Nitrotyrosine in Proben von zu detektieren.

Kurzübersicht für Nitrotyrosine Antikörper (ABIN126845)

Target: Nitrotyrosine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nitrotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: 7A5

Produktdetails anti-Nitrotyrosine Antikörper

Produktmerkmale: Synonyms: NO-Tyrosine, Nitro-Tyrosine

Aufreinigung: Subsequent Ultrafiltration and Size Exclusion Chromatography

Immunogen: KLH modified with Nitrotyrosine

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT)

Buffer: 1 mL2 x PBS, 0.09 % Sodium Azide, PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Nitrotyrosine

Target: Nitrotyrosine

Substanzklasse: Chemical

Hintergrund: The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). NO forms peroxynitrite following the reaction with superoxide. Peroxynitrite is a potent oxidant that reacts with a variety of biological molecules, including tyrosine residues in proteins. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosnine residues important for signal transduction.Synonyms: NO-Tyrosine, Nitro-Tyrosine