Insulin Receptor Antikörper (Beta Chain)

Produktnummer ABIN126820

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Insulin Receptor in WB, EIA und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Hund.

Kurzübersicht für Insulin Receptor Antikörper (Beta Chain) (ABIN126820)

Target: Insulin Receptor (INSR)

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Insulin Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 9H4

Produktdetails anti-Insulin Receptor Antikörper

Bindungsspezifität: Beta Chain

Spezifität: This antibody specifically recognizes the activation loop of Insulin receptor (phosphorylation-independent).

Produktmerkmale: Synonyms: Insulin Receptor

Aufreinigung: Size Exclusion Chromatography

Bestandteile: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated T47D cells. ELISA: 0.1 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Insulin Receptor

Target: Insulin Receptor (INSR)

Andere Bezeichnung: CD220 / INSR

Hintergrund: The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes.Synonyms: Insulin Receptor

Gen-ID: 3643

UniProt: P06213

Pathways: NF-kappaB Signalweg, RTK Signalweg, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity