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EGFR Antikörper (pTyr869)

Der Maus Monoklonal Anti-EGFR-Antikörper wurde für WB, IF, IHC (p), IP, EIA und IHC (fro) validiert. Er ist geeignet, EGFR in Proben von Human und Maus zu detektieren.
Produktnummer ABIN126761

Kurzübersicht für EGFR Antikörper (pTyr869) (ABIN126761)

Target

Alle EGFR Antikörper anzeigen
EGFR (Epidermal Growth Factor Receptor (EGFR))

Reaktivität

  • 853
  • 301
  • 289
  • 25
  • 25
  • 24
  • 20
  • 14
  • 8
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Maus

Wirt

  • 614
  • 240
  • 14
  • 13
  • 5
  • 4
  • 1
  • 1
  • 1
Maus

Klonalität

  • 546
  • 342
  • 2
Monoklonal

Konjugat

  • 467
  • 66
  • 28
  • 21
  • 18
  • 18
  • 18
  • 17
  • 16
  • 16
  • 16
  • 13
  • 13
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 9
  • 9
  • 9
  • 9
  • 9
  • 8
  • 8
  • 2
  • 1
  • 1
  • 1
Dieser EGFR Antikörper ist unkonjugiert

Applikation

  • 613
  • 265
  • 211
  • 169
  • 147
  • 128
  • 102
  • 92
  • 83
  • 82
  • 37
  • 33
  • 32
  • 27
  • 19
  • 11
  • 8
  • 6
  • 6
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon

12A3
  • Bindungsspezifität

    • 42
    • 39
    • 31
    • 31
    • 30
    • 29
    • 27
    • 27
    • 26
    • 22
    • 22
    • 18
    • 16
    • 16
    • 15
    • 15
    • 15
    • 15
    • 15
    • 13
    • 12
    • 12
    • 11
    • 10
    • 10
    • 9
    • 9
    • 9
    • 9
    • 8
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    pTyr869

    Spezifität

    Clone 12A3 specifically recognizes EGFR phosphorylated at Tyrosine 869 and detects EGFR activation after interaction with src kinases. Antibody AM00031PU-N does not crossreact with the highly homologues pTyr 877 of activated erbB2.

    Kreuzreaktivität (Details)

    Species reactivity (tested):Human, Mouse.

    Produktmerkmale

    Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

    Aufreinigung

    Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

    Bestandteile

    incl. positive Control

    Immunogen

    Phosphopeptide conjugated to KLHEpitope: Phosphotyrosine 869AA Sequence: E K E pY H A E

    Isotyp

    IgG1
  • Applikationshinweise

    ELISA: Use at 0.1 μg/mL (direct ELISA). Immunocytochemistry: Use at 1-10 μg/mL. Immunohistochemistry on Frozen and Paraffin Embedded Material. Immunoprecipitation: Use at 1-10 μg per 10^6 vanadate-treated A431 cells. Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer (cat#AS00002BU-N). Included Positive Control: Cell lysate delivered with this product (See Protocol below! ).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Protokoll

    Positive control cell lysate provided: HepG2 EGF Treated - recommended for immunoblottingFormulation: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. withEGF. Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Store in aliquots at -20°C. Avoidrepeated freezing and thawing. Applications: 20 µl of positive cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) forHRPO/ECL detection of the target proteins.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Rekonstitution

    Restore in 1 mL distilled water for 15 min at RT.

    Buffer

    PBS containing 0.09 % Sodium Azide / PEG and Sucrose.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution,aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at-80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3months. Avoid repeated freeze / thaw cycles!

    Haltbarkeit

    12 months
  • Target

    EGFR (Epidermal Growth Factor Receptor (EGFR))

    Andere Bezeichnung

    EGFR / ERBB1

    Hintergrund

    EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

    Gen-ID

    1956

    UniProt

    P00533

    Pathways

    NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine
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