CK1 epsilon Antikörper (AA 355-380)

Produktnummer ABIN126752

Der Maus Monoklonal anti-CK1 epsilon Antikörper wird verwendet zum Nachweis von CK1 epsilon in Proben von Human, Maus, Ratte und Hund. Er wurde validiert für WB und EIA.

Kurzübersicht für CK1 epsilon Antikörper (AA 355-380) (ABIN126752)

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CK1 epsilon Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: 4D7

Produktdetails anti-CK1 epsilon Antikörper

Bindungsspezifität: AA 355-380

Spezifität: Mab CK1e-4D7 specifically recognizes the epsilon isoform of CK1.

Produktmerkmale: Synonyms: CKI-epsilon, CKIe, CSNK1E

Aufreinigung: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

Bestandteile: incl. positive Control

Immunogen: Peptide (aa 355 - 380 of CK1e) conjugated to hemocyanin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA (0.1 μg/mL). Western blot (detects a band at 45 kD, Use at 0.5 μg/mL for HRPO/ECL detection,Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: For positive control cell lysate from serum starved A431 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Reconstitute with 1 mL H2O (15 min, RT)

Buffer: 2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For long term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution aliquoteand freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80 °C up to 1year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months. Avoidrepeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu CK1 epsilon

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Andere Bezeichnung: Casein Kinase I epsilon

Hintergrund: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as subtrates. It can also phosphorylate a large number of proteins and participates in Wnt signaling.Synonyms: CKI-epsilon, CKIe, CSNK1E

Gen-ID: 1454

UniProt: P49674

Pathways: Hedgehog Signalweg, M Phase