CK1 epsilon Antikörper (AA 355-380)

Produktnummer ABIN126752

Der Maus Monoklonal Anti-CK1 epsilon-Antikörper wurde für WB und EIA validiert. Er ist geeignet, CK1 epsilon in Proben von Human, Maus, Ratte und Hund zu detektieren.

Kurzübersicht für CK1 epsilon Antikörper (AA 355-380) (ABIN126752)

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CK1 epsilon Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: 4D7

Produktdetails anti-CK1 epsilon Antikörper

Bindungsspezifität: AA 355-380

Spezifität: Mab CK1e-4D7 specifically recognizes the epsilon isoform of CK1.

Produktmerkmale: Synonyms: CKI-epsilon, CKIe, CSNK1E

Aufreinigung: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

Bestandteile: incl. positive Control

Immunogen: Peptide (aa 355 - 380 of CK1e) conjugated to hemocyanin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA (0.1 μg/mL). Western blot (detects a band at 45 kD, Use at 0.5 μg/mL for HRPO/ECL detection,Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: For positive control cell lysate from serum starved A431 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Reconstitute with 1 mL H2O (15 min, RT)

Buffer: 2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For long term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution aliquoteand freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80 °C up to 1year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months. Avoidrepeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu CK1 epsilon

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Andere Bezeichnung: Casein Kinase I epsilon

Hintergrund: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as subtrates. It can also phosphorylate a large number of proteins and participates in Wnt signaling.Synonyms: CKI-epsilon, CKIe, CSNK1E

Gen-ID: 1454

UniProt: P49674

Pathways: Hedgehog Signalweg, M Phase