MAP2 Antikörper

Produktnummer ABIN125739

Produktdetails anti-MAP2 Antikörper

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reaktivität: Human, Maus, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Anti-MAP2 Purified

Spezifität: The antibody MT-08 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab), an intracellular antigen.

Kreuzreaktivität (Details): Human, Porcine, Mouse

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Microtubule protein (bovine brain) enriched for kinesin

Klon: MT-08

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: brain.
Immunohistochemistry (frozen sections): Positive tissue: murine brain.
Immunoprecipitation: Positive material: porcine brain.
Western blotting: Positive control: porcine brain.
ELISA: Positive control: porcine brain.
Immunocytochemistry: Positive control: human neuroblastoma SH-SY5Y.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (mIHC)

Validierung #104333 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104333

Datum: 20.04.2021

Antigen: MAP2

Chargennummer: 536958

Validierte Anwendung: Multiplex Immunohistochemistry

Positivkontrolle:

Fresh frozen mouse olfactory bulb

Negativkontrolle:

Unlabeled control (mouse fresh frozen)

Bewertung:

Passed. The anti-MAP2 antibody ABIN125739 specifically labels structural elements of neurons. Staining can be observed in neuronal cell bodies, axons and occasionally in proximal dendritic processes.

Validierungsbilder

Murine fresh frozen coronal olfactory bulb section (Thickness = 5 µm) labeled with anti-MAP2 antibody ABIN125739 (blue; bound to fluorophore ATTO 550). Labeling is present throughout olfactory bulb layers with and concentration in the external plexiform and mitral cell layers. Slc17a7 and PSD-95/DLG4 were labeled with ABIN1027710 (green; bound to fluorophore ATTO 550) and ABIN361694 (red; bound to fluorophore ATTO 550).

FFPE normal human cortex tissue section labeled with anti-MAP2 antibody ABIN125739 (cyan; bound to fluorophore ATTO 550) after EDTA antigen retrieval. DLG4 and Synapsin were labeled with anti-DLG4 antibody ABIN361694 (green; bound to fluorophore ATTO 550) and anti-SYN1 antibody ABIN5542390 (red; bound to fluorophore AF488).

Protokoll

Primärantikörper: ABIN125739

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-MAP2 antibody ABIN125739 to an oligo barcode used to bind oligo-bound fluorophore ATTO 550.

Anmerkungen:

• No signal was detected in unlabeled specimens.

• Specific staining of MAP2 was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-MAP2 Antikörper (ABIN125739)

Dzijak, Yildirim, Kahle, Novák, Hnilicová, Venit, Hozák: "Specific nuclear localizing sequence directs two myosin isoforms to the cell nucleus in calmodulin-sensitive manner." in: PLoS ONE, Vol. 7, Issue 1, pp. e30529, (2012) (PubMed).

Details zu MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Andere Bezeichnung: MAP2 (MAP2 Produkte)

Hintergrund: Microtubule associated protein 2,MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.,Microtubule-associated protein 2, MAP-2

Gen-ID: 4133

UniProt: P11137