Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

MAP2 Antikörper

Dieses Anti-MAP2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MAP2 in WB, ELISA, ICC, IP, IHC (p) und IHC (fro). Geeignet für Human, Maus und Schwein. Unabhängig validiert für die Verwendung in Multiplex Immunohistochemistry. Dieses Primary Antibody wurde in 1 Publikation zitiert.
Produktnummer ABIN125739

Kurzübersicht für MAP2 Antikörper (ABIN125739)

Target

Alle MAP2 Antikörper anzeigen
MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reaktivität

  • 149
  • 97
  • 93
  • 28
  • 24
  • 5
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Maus, Schwein

Wirt

  • 119
  • 52
  • 9
  • 2
Maus

Klonalität

  • 130
  • 51
Monoklonal

Konjugat

  • 101
  • 15
  • 13
  • 5
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser MAP2 Antikörper ist unkonjugiert

Applikation

  • 136
  • 75
  • 42
  • 40
  • 39
  • 39
  • 32
  • 31
  • 30
  • 21
  • 20
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon

MT-08
  • Verwendungszweck

    Anti-MAP2 Purified

    Spezifität

    The antibody MT-08 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab), an intracellular antigen.

    Kreuzreaktivität (Details)

    Human, Porcine, Mouse

    Aufreinigung

    Purified by protein-A affinity chromatography.

    Reinheit

    > 95 % (by SDS-PAGE)

    Immunogen

    Microtubule protein (bovine brain) enriched for kinesin

    Isotyp

    IgG1
  • Applikationshinweise

    Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: brain.
    Immunohistochemistry (frozen sections): Positive tissue: murine brain.
    Immunoprecipitation: Positive material: porcine brain.
    Western blotting: Positive control: porcine brain.
    ELISA: Positive control: porcine brain.
    Immunocytochemistry: Positive control: human neuroblastoma SH-SY5Y.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Validierung #104333 (Multiplex Immunohistochemistry)
    'Independent Validation' Siegel
    by
    Akoya Biosciences
    No.
    #104333
    Datum
    20.04.2021
    Antigen
    MAP2
    Chargennummer
    536958
    Validierte Anwendung
    Multiplex Immunohistochemistry
    Positivkontrolle

    Fresh frozen mouse olfactory bulb

    Negativkontrolle

    Unlabeled control (mouse fresh frozen)

    Bewertung

    Passed. The anti-MAP2 antibody ABIN125739 specifically labels structural elements of neurons. Staining can be observed in neuronal cell bodies, axons and occasionally in proximal dendritic processes.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN125739
    Sekundärantikörper
    Full Protocol
    • Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
    • Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
    • Conjugation of the anti-MAP2 antibody ABIN125739 to an oligo barcode used to bind oligo-bound fluorophore ATTO 550.
    Anmerkungen
    • No signal was detected in unlabeled specimens.

    • Specific staining of MAP2 was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

    • Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

  • Konzentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Do not freeze.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Do not freeze.
  • Dzijak, Yildirim, Kahle, Novák, Hnilicová, Venit, Hozák: "Specific nuclear localizing sequence directs two myosin isoforms to the cell nucleus in calmodulin-sensitive manner." in: PLoS ONE, Vol. 7, Issue 1, pp. e30529, (2012) (PubMed).

  • Target

    MAP2 (Microtubule-Associated Protein 2 (MAP2))

    Andere Bezeichnung

    MAP2

    Hintergrund

    Microtubule associated protein 2,MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.,Microtubule-associated protein 2, MAP-2

    Gen-ID

    4133

    UniProt

    P11137
Sie sind hier:
Chat with us!