UBE2N Antikörper (C-Term)

Produktnummer ABIN121903

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch UBE2N in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für UBE2N Antikörper (C-Term) (ABIN121903)

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBE2N Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-UBE2N Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Ion exchange chromatography

Immunogen: A 15 amino acid peptide located near the human UBC13 carboxy-terminus

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL - 1 μg/mL, this antibody detects a band of approximately 16 kDa in human small intestine cell lystates. Immunohistochemistry on frozen sections: 2 μg/mL, this product requires antigen retrieval using heat treatment prior to staining of paraffin sections, sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Mouse thymus tissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu UBE2N

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Hintergrund: UBC13 is a 16 kDa enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway. UBC13 is DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation.Synonyms: Bendless-like ubiquitin-conjugating enzyme, UBC13, Ubiquitin carrier protein N, Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N

UniProt: P61088

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway