LYN Antikörper (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog) (AA 8-238)

Details for Product anti-LYN Antibody No. ABIN120725
Antigen
  • JTK8
  • p53Lyn
  • p56Lyn
  • lyn
  • LYN
  • AA407514
  • Hck-2
  • jtk8
  • lyn-A
  • CH73-38P6.3
  • zgc:92124
  • LYN proto-oncogene, Src family tyrosine kinase
  • v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
  • tyrosine-protein kinase Lyn
  • LYN proto-oncogene, Src family tyrosine kinase L homeolog
  • LYN
  • lyn
  • Lyn
  • LOC100593961
  • lyn.L
Epitop
AA 8-238
25
16
16
15
11
11
9
8
8
7
6
6
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
245
145
112
19
16
6
5
4
4
3
3
3
2
2
1
Wirt
Maus
206
36
17
3
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser LYN Antikörper ist unkonjugiert
13
10
7
6
5
5
4
4
4
4
4
4
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
212
112
101
37
28
17
14
11
10
8
4
3
1
Optionen
Immunogen Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238)
Klon LYN-01
Isotyp IgG1
Spezifität The antibody reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues.
Reinigung Protein A affinity chromatography
Plasmids, Primers & others Plasmide, Primers & weitere LYN products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung LYN (LYN Antibody Abstract)
Hintergrund Lyn (also known as p53/56 Lyn) is a membrane associated protein tyrosine kinase (PTK) mostly expressed in hemopoietic cells which is important in cellular signaling. It contains an SH2 and SH3 domain and has been found to be cleaved after activation of caspases in apoptosis. A member of the Src family of PTKs, there are two known isoforms for Lyn which plays an indispensable role in the Fc epsilon RI (Fcer1) and the B cell IgM receptor signaling pathway and is essential for Syk activation and Lat phosphorylation after Fcer1 aggregation and can also phosphorylate Tec on multiple residues. Lyn can also be regulated by IL2 and IL3. Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).Synonyms: Tyrosine-protein kinase Lyn
Gen-ID 4067
UniProt P07948
Pathways Fc-epsilon Rezeptor Signalübertragung, Hormone Transport, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling, Integrin Complex
Applikations-hinweise Immunoprecipitation. Immunocytochemistry. Western Blotting: Recommended dilution: 1-2 μg/mL, 60 min. Positive control: RBL rat basophilic leukemia cell line JURKAT human peripheral blood leukemia T-cell line A-431 human epidermoid carcinoma cell line U-937 human histiocytic lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate (1:1) with non- reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (12 % separating gel).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer PBS, pH ~7.4, 15 mM Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Haltbarkeit 12 months
Produkt verwendet in: Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Kovárová, Tolar, Arudchandran, Dráberová, Rivera, Dráber: "Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation." in: Molecular and cellular biology, Vol. 21, Issue 24, pp. 8318-28, 2001 (PubMed).

Amoui, Dráberová, Tolar, Dráber: "Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors." in: European journal of immunology, Vol. 27, Issue 1, pp. 321-8, 1997 (PubMed).

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