KIF5B Antikörper

Produktnummer ABIN120715

Dieses Maus Monoklonal-Antikörper erkennt spezifisch KIF5B in IF. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Schwein.

Kurzübersicht für KIF5B Antikörper (ABIN120715)

Target: KIF5B (Kinesin Family Member 5B (KIF5B))

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KIF5B Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF)

Klon: KN-02

Produktdetails anti-KIF5B Antikörper

Spezifität: The antibody KN-02 recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.

Aufreinigung: Thiophilic adsorption-affinity chromatography

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Enriched fraction of porcine brain kinesin

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Details zu KIF5B

Target: KIF5B (Kinesin Family Member 5B (KIF5B))

Andere Bezeichnung: KIF5B

Hintergrund: Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.Synonyms: Conventional kinesin heavy chain, KIF5B, KNS, KNS1, Kinesin-1 heavy chain, UKHC, Ubiquitous kinesin heavy chain

Gen-ID: 3799

UniProt: P33176

Pathways: Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization