Integrin beta 2 Antikörper

Produktnummer ABIN120700

Produktdetails anti-Integrin beta 2 Antikörper

Target: Integrin beta 2 (ITGB2)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Integrin beta 2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: The antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (Integrin beta2 chain). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Leucocytes of a patient suffering from a LGL-type leukaemia

Klon: MEM-48

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Immunoprecipitation. Western Blotting (2 μg/mL): Positive control: Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. Application note: Non-reducing conditions. SDS-PAGE (6 % separating gel, 4 % stacking gel). Immunohistochemistry (frozen section). Functional application: The antibody induces high-affinity conformation of LFA-1 complex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4 with 15 mM Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. General.

Haltbarkeit: 12 months

Details zu Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Andere Bezeichnung: CD18 / ITGB2 (ITGB2 Produkte)

Hintergrund: CD18, integrin b2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gen-ID: 3689

UniProt: P05107

Pathways: NF-kappaB Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation