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Blood Group Lewis B Antikörper

Der Maus Monoklonal Anti-Blood Group Lewis B-Antikörper wurde für IHC (p), WB und EIA validiert. Er ist geeignet, Blood Group Lewis B in Proben von Human zu detektieren.
Produktnummer ABIN120686

Kurzübersicht für Blood Group Lewis B Antikörper (ABIN120686)

Target

Blood Group Lewis B

Reaktivität

  • 14
  • 12
  • 10
Human

Wirt

  • 14
Maus

Klonalität

  • 14
Monoklonal

Konjugat

  • 6
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Blood Group Lewis B Antikörper ist unkonjugiert

Applikation

  • 12
  • 9
  • 9
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon

2-25LE
  • Kreuzreaktivität (Details)

    Species reactivity (tested):Human.

    Aufreinigung

    Affinity Chromatography on Protein A.

    Immunogen

    Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification)

    Isotyp

    IgG1
  • Applikationshinweise

    Immunohistochemistry: 2-4 μg/mL for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Use human colon CA for postive control. Western blot. ELISA.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1.0 mg/mL

    Buffer

    PBS, pH 7.4, with 15 mM Sodium Azide as preservative.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store at 2-8 °C for 6 months. For long-term storage aliquot and store at -20 °C. Avoid freeze/thaw cycles.
    Shelf Life: one year from despatch.

    Haltbarkeit

    12 months
  • Target

    Blood Group Lewis B

    Hintergrund

    Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.Synonyms: Blood Group Antigen Lewis b
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