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Blood Group Lewis B Antikörper (CF®594)

Reaktivität: Human, Meerschweinchen, Schwein IF, IHC (f), ISt Wirt: Maus Monoclonal 2-25LE CF®594
Produktnummer ABIN6153097
  • Target Alle Blood Group Lewis B Produkte
    Blood Group Lewis B
    Reaktivität
    Human, Meerschweinchen, Schwein
    Wirt
    • 13
    Maus
    Klonalität
    • 13
    Monoklonal
    Konjugat
    • 7
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Blood Group Lewis B Antikörper ist konjugiert mit CF®594
    Applikation
    • 11
    • 8
    • 8
    • 5
    • 2
    • 2
    • 1
    • 1
    Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)
    Verwendungszweck
    Mouse Monoclonal anti-Lewis-B (2-25LE), CF594 Conjugate
    Produktmerkmale
    The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Mucin isolated from a human ovarian cyst fluid
    Klon
    2-25LE
    Isotyp
    IgG1 kappa
  • Applikationshinweise
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Kommentare

    Colon

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Protect from light
  • Target
    Blood Group Lewis B
    Andere Bezeichnung
    Lewis-B (Blood Group Lewis B Produkte)
    Synonyme
    MUC5 antikoerper, TBM antikoerper, leB antikoerper, mucin 5AC, oligomeric mucus/gel-forming antikoerper, MUC5AC antikoerper
    Molekulargewicht
    45 kDa
    Gen-ID
    2525, 169238
    UniProt
    P21217
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