Blood Group Lewis B Antikörper (CF®647)

Produktnummer ABIN6153081

Produktdetails anti-Blood Group Lewis B Antikörper (CF®647)

Target: Blood Group Lewis B

Reaktivität: Human, Meerschweinchen, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Blood Group Lewis B Antikörper ist konjugiert mit CF®647

Applikation: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

Verwendungszweck: Mouse Monoclonal anti-Lewis-B (2-25LE), CF647 Conjugate

Produktmerkmale: The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Mucin isolated from a human ovarian cyst fluid

Klon: 2-25LE

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Colon

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Blood Group Lewis B

Target: Blood Group Lewis B

Andere Bezeichnung: Lewis-B (Blood Group Lewis B Produkte)

Synonyme: MUC5 antikoerper, TBM antikoerper, leB antikoerper, mucin 5AC, oligomeric mucus/gel-forming antikoerper, MUC5AC antikoerper

Molekulargewicht: 45 kDa

Gen-ID: 2525, 169238

UniProt: P21217