CD22 Antikörper
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- Target Alle CD22 Antikörper anzeigen
- CD22 (CD22 Molecule (CD22))
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Reaktivität
- Human, Primate
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD22 Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
- Kreuzreaktivität (Details)
- Species reactivity (tested):Human, non-human Primates
- Aufreinigung
- Protein-A affinity chromatography
- Reinheit
- > 95 % pure (by SDS-PAGE)
- Immunogen
- Raji Burkitt's lymphoma cell line
- Klon
- MEM-01
- Isotyp
- IgG1
- Top Product
- Discover our top product CD22 Primärantikörper
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anti-CD22 Molecule (CD22) (AA 621-725) antibody
CD22 Reaktivität: Human FACS, WB, ELISA, IHC, ICC Wirt: Maus Monoclonal 2H1C4 unconjugated
anti-CD22 Molecule (CD22) (AA 621-725) antibodyCD22 Reaktivität: Human FACS, ELISA, ICC Wirt: Maus Monoclonal 1A3A11 unconjugated
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 Reaktivität: Human FACS, WB, ELISA, IHC, IF, Coat, StM Wirt: Maus Monoclonal BLCAM-1795 unconjugated
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 Reaktivität: Human FACS, WB, ELISA, IHC, IF, Coat Wirt: Maus Monoclonal BLCAM-1796 unconjugated
anti-CD22 Molecule (CD22) antibodyCD22 Reaktivität: Human, Non-Human Primate FACS, WB, IP Wirt: Maus Monoclonal MEM-01 unconjugated
anti-CD22 Molecule (CD22) antibody (PE)CD22 Reaktivität: Human, Non-Human Primate FACS Wirt: Maus Monoclonal MEM-01 PE
anti-CD22 Molecule (CD22) antibody (FITC)CD22 Reaktivität: Human FACS Wirt: Maus Monoclonal IS7 FITC
anti-CD22 Molecule (CD22) (AA 708-847) antibodyCD22 Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal unconjugated
anti-CD22 Molecule (CD22) antibodyCD22 Reaktivität: Human, Maus, Ratte, Affe, Hund WB, IHC, IHC (p) Wirt: Maus Monoclonal 4C3 unconjugated
anti-CD22 Molecule (CD22) (AA 20-687) antibodyCD22 Reaktivität: Human FACS, WB, ELISA, IF, IP Wirt: Kaninchen Monoclonal DM13 unconjugated
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- Applikationshinweise
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Flow cytometry. Immunoprecipitation. Western blotting: Recommended dilution: 2-4 μg/mL, 120 min on vertical incubator Positive control: RAJI human lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. - Haltbarkeit
- 12 months
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- Target
- CD22 (CD22 Molecule (CD22))
- Andere Bezeichnung
- CD22 (CD22 Produkte)
- Hintergrund
- CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.Synonyms: B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Leu-14, SIGLEC2, Sialic acid-binding Ig-like lectin 2, Siglec-2
- UniProt
- P20273
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