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Cytokeratin 18 Antikörper

Der Maus Monoklonal Anti-Cytokeratin 18-Antikörper wurde für WB, IF, IHC (p), IP und EIA validiert. Er ist geeignet, Cytokeratin 18 in Proben von Human zu detektieren. Es sind 7+ Publikationen verfügbar.
Produktnummer ABIN120626

Kurzübersicht für Cytokeratin 18 Antikörper (ABIN120626)

Target

Alle Cytokeratin 18 (KRT18) Antikörper anzeigen
Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reaktivität

  • 332
  • 155
  • 130
  • 76
  • 67
  • 58
  • 53
  • 50
  • 42
  • 39
  • 35
  • 35
  • 35
  • 18
  • 8
  • 8
  • 7
  • 6
  • 4
  • 3
  • 2
  • 1
Human

Wirt

  • 192
  • 182
  • 3
  • 1
  • 1
  • 1
Maus

Klonalität

  • 231
  • 146
  • 3
Monoklonal

Konjugat

  • 195
  • 30
  • 23
  • 13
  • 10
  • 7
  • 7
  • 7
  • 7
  • 7
  • 7
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Dieser Cytokeratin 18 Antikörper ist unkonjugiert

Applikation

  • 315
  • 193
  • 123
  • 122
  • 73
  • 69
  • 59
  • 58
  • 54
  • 45
  • 40
  • 32
  • 19
  • 8
  • 8
  • 7
  • 6
  • 4
  • 2
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Klon

DA-7
  • Spezifität

    The antibody DA-7 reacts with Cytokeratin 18 (45 kDa).

    Kreuzreaktivität (Details)

    Species reactivity (tested):Human.

    Aufreinigung

    Affinity Chromatography on Protein A

    Immunogen

    Human breast carcinoma cell line PMC-42

    Isotyp

    IgG1
  • Applikationshinweise

    Immunoprecipitation. Western Blot: 1-2 mg/mL, overnight in 4 °C, Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail), Incubate 60 min on ice, Centrifuge to remove cell debris, Mix lysate with non-reducing SDS-PAGE sample buffer. Positive control: HeLa human cervix carcinoma cell line, A-431 human epidermoid carcinoma cell line, MCF-7 human breast adenocarcinoma cell line. Immunohistochemistry on paraffin sections: 1:10 - 1:100. Positive tissue: epithelial tissue (skin). Immunocytochemistry. Positive control: MCF-7 human breast adenocarcinoma cell line HeLa human cervix carcinoma cell line. ELISA: The antibody DA-7 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04. Positive control: MCF-7 human breast adenocarcinoma cell line.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1.0 mg/mL

    Buffer

    PBS, pH ~7.4, 15 mM Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf Life: one year from despatch.

    Haltbarkeit

    12 months
  • Kaulfürst-Soboll, Mertens, Brehler, von Schaewen: "Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis." in: PLoS ONE, Vol. 6, Issue 3, pp. e17800, (2011) (PubMed).

    Cebecauer, Cerný, Horejsí: "Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells." in: Biochemical and biophysical research communications, Vol. 243, Issue 3, pp. 706-10, (1998) (PubMed).

    Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, (1990) (PubMed).

    Vojt?sek, Stasková, Nenutil, Bártková, Kovarík, Rejthar, Bártek: "A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis." in: Neoplasma, Vol. 37, Issue 3, pp. 333-42, (1990) (PubMed).

    Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).

    Horejsí, N?mec, Angelisová, Kristofová, Gorga, Hilgert: "Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens." in: Tissue antigens, Vol. 28, Issue 5, pp. 288-97, (1987) (PubMed).

    Horejsí, Hilgert, Kristofová, Bazil, Bukovský, Kulhánková et al.: "Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte ..." in: Folia biologica, Vol. 32, Issue 1, pp. 12-25, (1986) (PubMed).

  • Target

    Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

    Andere Bezeichnung

    Cytokeratin 18

    Hintergrund

    Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18

    Gen-ID

    3875

    UniProt

    P05783

    Pathways

    Apoptose, Caspase Kaskade in der Apoptose
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