Integrin beta 2 Antikörper

Produktnummer ABIN120420

Der Maus Monoklonal anti-Integrin beta 2 Antikörper (Klon WT-3) (ABIN120420) detektiert spezifisch Integrin beta 2 in FACS, WB, IP und Func. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für Integrin beta 2 Antikörper (ABIN120420)

Target: Integrin beta 2 (ITGB2)

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Integrin beta 2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)

Klon: WT-3

Produktdetails anti-Integrin beta 2 Antikörper

Spezifität: This antibody reacts with the beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits. Clone WT.3 has been shown to inhibit homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1. SM264A is recommended for this purpose.

Aufreinigung: Affinity chromatography on Protein G

Immunogen: FTL-43. Lymph node cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Andere Bezeichnung: CD18 / ITGB2

Hintergrund: CD18 is expressed by all leucocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gen-ID: 25021

NCBI Accession: NP_036843

UniProt: Q9JI30

Pathways: NF-kappaB Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation