Aurora A Antikörper

Produktnummer ABIN119942

Der Maus Monoklonal anti-Aurora A Antikörper wird verwendet zum Nachweis von Aurora A in Proben von Human und Maus. Er wurde validiert für WB, IF und IP.

Kurzübersicht für Aurora A Antikörper (ABIN119942)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Aurora A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 35C1

Produktdetails anti-Aurora A Antikörper

Spezifität: This antibody recognises Aurora-A kinase. Clone 35C1 specifically recognises an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity.

Aufreinigung: Affinity chromatography on Protein G

Immunogen: Recombinant Aurora-A. Spleen cells from immunised Balb/c were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation. Western Blot: 1/500 - 1/1000, this antibody detects a band of approximately 46 kDa in human HeLa and mouse M-ICc12 cell lysates. Immunofluorescence. Recommended Positive Control: Human 293 and mouse LLC1 cell lines.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Andere Bezeichnung: Aurora Kinase A

Hintergrund: Aurora-A kinase is a 46 kDa serine/threonine kinase involved in mitotic spindle assembly. Aurora-A is reported to be overexpressed in many epithelial cancers and is thought to play an important role in tumourigenesis.Synonyms: AIK1, ARK1, AURA, AURKA, AURORA2, Aurora/IPL1-related kinase 1, BTAK, Breast tumor-amplified kinase, STK15, STK6, Serine/threonine kinase 15, Serine/threonine-protein kinase 6

Gen-ID: 6790

NCBI Accession: NP_003591

UniProt: O14965

Pathways: Zellzyklus, Asymmetric Protein Localization