GZMB Antikörper

Produktnummer ABIN119565

Dieser Anti-GZMB-Antikörper ist ein Maus-Monoklonal-Antikörper zum Nachweis von GZMB in FACS, IF, IP, IHC (fro) und EIA. Geeignet für Human, Affe und Schimpanse.

Kurzübersicht für GZMB Antikörper (ABIN119565)

Target: GZMB (Granzyme B (GZMB))

Reaktivität: Human, Affe, Schimpanse

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GZMB Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: GB11

Produktdetails anti-GZMB Antikörper

Spezifität: This antibody recognises the serine protease Granzyme B. This antibody has been reported to function as a Capture reagent in ELISA assays for soluble Granzyme B in conjunction with biotin conjugated clone GB10 (SM1805B) as detection reagent.

Aufreinigung: Affinity chromatography on Protein A

Immunogen: Purified human Granzyme B. Remarks: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen sections. Flow cytometry: 1/10 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood, Membrane permeabilisation is required for this application. Immunoprecipitation. ELISA: 2 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Tris buffered saline containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GZMB

Target: GZMB (Granzyme B (GZMB))

Hintergrund: Ganzyme B is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs). Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death.Synonyms: CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, Granzyme-2, Lymphocyte protease, SECT, T-cell serine protease 1-3E

Gen-ID: 3002

NCBI Accession: NP_004122

UniProt: P10144

Pathways: Apoptose, Caspase Kaskade in der Apoptose