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CD3 Antikörper (Cytoplasmic Domain)

CD3 Reaktivität: Human, Maus, Huhn, Schwein, Hund, Pferd FACS, IHC (fro), IF, WB, IHC (p) Wirt: Ratte Monoclonal CD3-12 unconjugated
Produktnummer ABIN119451
  • Target Alle CD3 Antikörper anzeigen
    CD3
    Bindungsspezifität
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reaktivität
    • 222
    • 96
    • 31
    • 14
    • 14
    • 10
    • 8
    • 7
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    Human, Maus, Huhn, Schwein, Hund, Pferd
    Wirt
    • 258
    • 51
    • 14
    • 13
    • 10
    Ratte
    Klonalität
    • 334
    • 8
    Monoklonal
    Konjugat
    • 97
    • 36
    • 32
    • 26
    • 24
    • 13
    • 9
    • 9
    • 9
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD3 Antikörper ist unkonjugiert
    Applikation
    • 294
    • 93
    • 58
    • 52
    • 45
    • 44
    • 38
    • 24
    • 16
    • 10
    • 9
    • 9
    • 8
    • 7
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Sequenz
    Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys
    Spezifität
    This antibody recognises a highly conserved epitope of the CD3 Molecule expressed by T lymphocytes. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross reactivity for the CD3 marker. (Jones et al. 1993, Kothlow et al. 2005).
    Kreuzreaktivität (Details)
    Species reactivity (expected):Mammals, Birds.
    Species reactivity (tested):Human, Mouse, Canine, Chicken, Equine, Porcine and Rhesus Monkey, Alpaca, Amazon Parrot, Duck, Harbour Porpoise, Koala, Raccoon, Sea Lion, Spotted Hyena.
    Aufreinigung
    Affinity Chromatography on Protein G
    Immunogen
    Synthetic peptide sequence derived from Cytoplasmic epitope of CD3
    Klon
    CD3-12
    Isotyp
    IgG1
    Top Product
    Discover our top product CD3 Primärantikörper
  • Applikationshinweise
    Flow cytometry (1/50-1/100, requires membrane permeabilisation). Immunohistochemistry on frozen sections and paraffin embedded tissues (1/1000). This product requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. 1 mM EDTA pH 8.0 is recommended for this purpose. Recommended positive control tissue: Tonsil. Western blotting.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    PBS, 0.09 % Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store the reconstituted antibody undiluted at 2-8 °C.
  • Hebel, Eisinger, Neumeier, Rein-Fischboeck, Pohl, Meier, Boettcher, Froehner, Adams, Liebisch, Krautbauer, Buechler: "Lipid abnormalities in alpha/beta2-syntrophin null mice are independent from ABCA1." in: Biochimica et biophysica acta, Vol. 1851, Issue 5, pp. 527-36, (2015) (PubMed).

    Hong, Puaux, Huang, Loumagne, Tow, Mackay, Kato, Prévost-Blondel, Avril, Nardin, Abastado: "Chemotherapy induces intratumoral expression of chemokines in cutaneous melanoma, favoring T-cell infiltration and tumor control." in: Cancer research, Vol. 71, Issue 22, pp. 6997-7009, (2011) (PubMed).

    Shrestha, Hashiguchi, Ito, Miura, Takenouchi, Oyama, Kawahara, Tancharoen, Ki-I, Arimura, Yoshinaga, Noma, Shrestha, Nitanda, Kitajima, Arimura, Sato, Sakamoto, Maruyama: "B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4819-26, (2010) (PubMed).

  • Target
    CD3
    Andere Bezeichnung
    CD3 (CD3 Produkte)
    Synonyme
    CD3 antikoerper, AI504783 antikoerper, CD3epsilon antikoerper, T3e antikoerper, cd3-gamma antikoerper, cd3g antikoerper, cd3g-a antikoerper, cd3g/d antikoerper, cd3gd antikoerper, t3g antikoerper, CD3e molecule antikoerper, CD3d molecule antikoerper, CD3 antigen, epsilon polypeptide antikoerper, CD3g molecule S homeolog antikoerper, CD3E antikoerper, CD3D antikoerper, Cd3e antikoerper, cd3g.S antikoerper
    Hintergrund
    T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
    Gen-ID
    916
    NCBI Accession
    NP_000724
    UniProt
    P07766
    Pathways
    T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway
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