HLAG Antikörper

Produktnummer ABIN119228

Der Maus Monoklonal anti-HLAG Antikörper wird verwendet zum Nachweis von HLAG in Proben von Human. Er wurde validiert für WB und IHC (p).

Kurzübersicht für HLAG Antikörper (ABIN119228)

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HLAG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: MEM-G-1

Produktdetails anti-HLAG Antikörper

Spezifität: The antibody reacts with denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.

Aufreinigung: Affinity Chromatography on Protein-A

Immunogen: Recombinant Human HLA-G denaturated heavy chain

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blotting. Immunohistochemistry (paraffin sections): 1:60 to 1:100, 1 h at RT. Positive tissue: Human placenta - extravillous cytotrophoblast. Staining technique: Standard ABC technique. Pretreatment: Heat retrieval in 0.01 M citrate buffer (4x2 min. in microwave oven).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to - 70 °C for longer.

Details zu HLAG

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Andere Bezeichnung: HLA Class I alpha G / HLA-G

Hintergrund: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.0, HLAG, MHC class I antigen G

Gen-ID: 3135

NCBI Accession: NP_002118

UniProt: P17693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response