PARP1 Antikörper

Produktnummer ABIN118984

Dieser Maus Monoklonal Antikörper detektiert spezifisch PARP1 in WB, IP, IHC (p), IHC (fro) und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Drosophila melanogaster, Hamster und Xenopus laevis.

Kurzübersicht für PARP1 Antikörper (ABIN118984)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reaktivität: Human, Maus, Ratte, Drosophila melanogaster, Hamster, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PARP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: A6-4-12

Produktdetails anti-PARP1 Antikörper

Spezifität: This antibody recognises poly (ADP-ribose) polymerase (PARP).

Kreuzreaktivität (Details): Species reactivity (expected):Xenopus, Drosophila, Hamster, Rat and Mouse.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Human PARP. Spleen cells from immunised BALB/c mice were fused with cells of mouse NSO myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. ELISA. Immunoprecipitation. Western Blot: 1/1000 - 1/5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Details zu PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Andere Bezeichnung: PARP1

Hintergrund: PARP (Poly (ADP-ribose) polymerase) is a 113 kDa nuclear protein which can exist as a homo- or hetero-dimer, and is strongly activated by DNA strand breaks. This protein acts as a molecular ''nick sensor'' and functions in base excision repair, poly(ADPribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism, and participates in protein modification to enhance or repress transcription. PARP also plays a role in other cellular processes, including cell proliferation and differentiation. PARP is ribosylated by PARP2, and during apoptosis, ICE family members, such as caspase 3 and 7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3.Synonyms: ADPRT, ADPRT 1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PPOL, Poly [ADP-ribose] polymerase 1, Poly[ADP-ribose] synthetase 1

Gen-ID: 142

NCBI Accession: NP_001609

UniProt: P09874

Pathways: Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location