CD40 Ligand Antikörper

Produktnummer ABIN118902

Dieser Anti-CD40 Ligand Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von CD40 Ligand in FACS. Geeignet für Human.

Kurzübersicht für CD40 Ligand Antikörper (ABIN118902)

Target: CD40 Ligand (CD40LG)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD40 Ligand Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Klon: TRAP1-3-6

Produktdetails anti-CD40 Ligand Antikörper

Spezifität: This antibody recognises the CD154 cell surface antigen, a 32 kDa glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4 +ve and also on some basophils and mast cells. This antibody binds to CD154 at an epitope distinct from the CD40 binding site.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Mouse myeloma cell line transfected with human CD40L (CD154). Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63.Ag 8653 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide, 5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD40 Ligand

Target: CD40 Ligand (CD40LG)

Andere Bezeichnung: CD154 / CD40L

Hintergrund: CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes. The protein encoded by this gene is a member of the TNF receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5

Gen-ID: 959

NCBI Accession: NP_000065

UniProt: P29965

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints