HLA-ABC Antikörper

Produktnummer ABIN118899

Dieses Anti-HLA-ABC-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von HLA-ABC in FACS, IP, IHC (fro), IF, Func, EIA und DNA Mic. Geeignet für Human, Rind (Kuh), Pavian, Makake, Cat, Affe, Primate und Schimpanse. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für HLA-ABC Antikörper (ABIN118899)

Target: HLA-ABC

Reaktivität: Human, Rind (Kuh), Pavian, Makake, Cat, Affe, Primate, Schimpanse

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HLA-ABC Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Functional Studies (Func), Enzyme Immunoassay (EIA), DNA Microscopy (DNA Mic)

Klon: W6-32

Produktdetails anti-HLA-ABC Antikörper

Aufreinigung: Affinity Chromatography on Protein G

Endotoxin-Niveau: Less than 0.01 EU/μg

Immunogen: Purified Human tonsil lymphocyte membranes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: This product is routinely tested in flow cytometry on human peripheral blood lymphocytes: Use 10 μL of 1/50 - 1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections (see Ref 6, Tonsil is useful as positive control). Not suitable for Immunohistochemistry on paraffin sections. Immunoprecipitation (Ref 1). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS without preservatives or stabilizers.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Referenzen für anti-HLA-ABC Antikörper (ABIN118899)

Giersch, Hermanussen, Volz, Volmari, Allweiss, Sureau, Casey, Huang, Fischer, Lütgehetmann, Dandri: "Strong Replication Interference Between Hepatitis Delta Viruses in Human Liver Chimeric Mice." in: Frontiers in microbiology, Vol. 12, pp. 671466, (2021) (PubMed).

Stern, Goldman, Hatasaka, Mackenzie, Surrey, Racowsky,: "Optimizing the number of cleavage stage embryos to transfer on day 3 in women 38 years of age and older: a Society for Assisted Reproductive Technology database study." in: Fertility and sterility, (2008) (PubMed).

Mayr, Bund, Schlee, Moosmann, Kofler, Hallek, Wendtner: "Fibromodulin as a novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (CLL), which allows expansion of specific CD8+ autologous T lymphocytes." in: Blood, Vol. 105, Issue 4, pp. 1566-73, (2005) (PubMed).

Mayr, Kofler, Büning, Bund, Hallek, Wendtner: "Transduction of CLL cells by CD40 ligand enhances an antigen-specific immune recognition by autologous T cells." in: Blood, Vol. 106, Issue 9, pp. 3223-6, (2005) (PubMed).

Details zu HLA-ABC

Target: HLA-ABC

Andere Bezeichnung: HLA Class I ABC

Hintergrund: Synonyms: HLA Class 2 ABC, Human Leukocyte antigen class I ABC, MHC class I ABC, Major Histocompatibility complex class I

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints