CD79a Antikörper

Produktnummer ABIN118796

Der Maus Monoklonal Anti-CD79a-Antikörper wurde für FACS, IP und EIA validiert. Er ist geeignet, CD79a in Proben von Human zu detektieren.

Kurzübersicht für CD79a Antikörper (ABIN118796)

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD79a Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Klon: ZL7-4

Produktdetails anti-CD79a Antikörper

Spezifität: This antibody reacts with CD79a positive cells by flow cytometry and ELISA specific for a fusion protein of CD79a-Fc. This clone has been reported to be useful in distinguishing B-CLL from mantle cell lymphoma in flow cytometric assays. (2) The antibody has been reported to induce phosphorylation of syk kinase. (3)

Aufreinigung: Affinity Chromatography on Protein A.

Immunogen: IgM complex isolated from Daudi cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of 10 μg/mL diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation: 20 μg/mL. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD79a

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Andere Bezeichnung: CD79a

Hintergrund: A disulfide-linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, is non-covalently associated with membrane bound immunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling.Synonyms: B-Cell marker, B-cell antigen receptor complex-associated protein alpha-chain, IGA, MB-1 membrane glycoprotein, MB1, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein

Gen-ID: 973

NCBI Accession: NP_001774

UniProt: P11912

Pathways: BCR Signaling