CD55 Antikörper

Produktnummer ABIN118737

Produktdetails anti-CD55 Antikörper

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD55 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: K562 cells

Klon: 67

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of 1/10-1/25 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections: 1/100-1/1000. Recommended Positive Control Tissue: Human Tonsil. (Successful use in immunohistochemistry on paraffin embedded sections (1/50-1/100using heat treatment for antigen retrieval) was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, with 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-CD55 Antikörper (ABIN118737)

Wenzel, Zabojszcza, Carl, Taubert, Lass, Harris, Ho, Schulz, Hummel, Hubner, Osterziel, Spuler: "Increased susceptibility to complement attack due to down-regulation of decay-accelerating factor/CD55 in dysferlin-deficient muscular dystrophy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 9, pp. 6219-25, (2005) (PubMed).

Details zu CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Andere Bezeichnung: CD55 / DAF (CD55 Produkte)

Synonyme: CR antikoerper, CROM antikoerper, DAF antikoerper, TC antikoerper, Daf antikoerper, Daf-GPI antikoerper, Daf1 antikoerper, GPI-DAF antikoerper, CD55 molecule (Cromer blood group) antikoerper, CD55 molecule, decay accelerating factor for complement antikoerper, CD55 antikoerper, Cd55 antikoerper

Hintergrund: CD55 is the complement regulatory protein, decay accelerating factor (DAF). It is a 70kD glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-haemopoietic tissues, particularly epithelium and is specifically found at the foetal-maternal interface in placenta. Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin. CD55 is expressed by a wide range of cell types.Synonyms: Complement decay-accelerating factor

Gen-ID: 9606

UniProt: P08174

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity