CD53 Antikörper

Produktnummer ABIN118729

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD53 in FACS, WB, IP, IHC (fro) und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD53 Antikörper (ABIN118729)

Target: CD53

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD53 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: MEM-53

Produktdetails anti-CD53 Antikörper

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1/10 - 1/25, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections. Western blotting under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD53

Target: CD53

Andere Bezeichnung: CD53

Hintergrund: CD53 is a member of the tetraspan family of molecules, and is expressed by all leucocytes, but is absent from red cells and platelets. Most of the tetraspan family of molecules are cell surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2 generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. CD53 is a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.Synonyms: Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, TSPAN25, Tetraspanin-25

Gen-ID: 963

NCBI Accession: NP_000551

UniProt: P19397