Sialoadhesin/CD169 Antikörper (FITC)

Produktnummer ABIN118291

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch Sialoadhesin/CD169 in FACS und IF. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für Sialoadhesin/CD169 Antikörper (FITC) (ABIN118291)

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Sialoadhesin/CD169 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: MOMA-1

Produktdetails anti-Sialoadhesin/CD169 Antikörper (FITC)

Spezifität: This antibody is specific for CD169, also known as Sialoadhesin. It reacts with a subpopulation of mature resident tissue macrophages. No reactivity is seen with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells or blood cells. Distinct macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localised at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localised in the sinusoids and medullary cords but not within follicular areas or paracortex. In Peyers patches they are localised in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1 although antigen expression is weaker than that seen in splenic macrophages. No MOMA-1 positive macrophages were found in the thymus. Reactivity was also negative in following organs tested so far (kidney, brain, skin). In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Stromal (reticular) elements from spleen.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Sialoadhesin/CD169

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Andere Bezeichnung: CD169 / SIGLEC1

Hintergrund: Two families of mammalian lectin like adhesion molecules have been shown to bind glycoconjugate ligands in a sialic acid dependent manner: the selectins and the sialoadhesins. The sialoadhesin family has 4 members: CD22, a B cell specific marker, myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells, CD33, a myeloid differentiation antigen, and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.Synonyms: Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec-1

Gen-ID: 20612

NCBI Accession: NP_035556

UniProt: Q62230