CD3 Antikörper (PE)

Produktnummer ABIN118107

Dieser Anti-CD3 Antikörper ist ein Ratte Monoklonal Antikörper zur Detektion von CD3 in FACS. Geeignet für Maus.

Kurzübersicht für CD3 Antikörper (PE) (ABIN118107)

Target: CD3

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: KT3

Produktdetails anti-CD3 Antikörper (PE)

Spezifität: This antibody recognises the CD3 antigen which is expressed by mature T cells.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells. For optimal stainingincubations should be performed at room temperature, The Fc region of monoclonalantibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL distilled water.

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 5 % Sucrose, 1 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3

Hintergrund: Cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gen-ID: 12501

NCBI Accession: NP_031674

UniProt: P22646

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway