CD3 Antikörper (APC)

Produktnummer ABIN118100

Produktdetails anti-CD3 Antikörper (APC)

Target: CD3

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Spezifität: This antibody recognises the CD3 antigen which is expressed by mature T cells.

Aufreinigung: Affinity chromatography on Protein A

Immunogen: Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.

Klon: KT3

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: For optimal staining incubations should be performed at roomtemperature, The Fc region of monoclonal antibodies may bind non-specifically to cellsexpressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL distilled water

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: Use of Sodium Szide as a preservative will substantially inhibit the enzyme activity of Horseradish Peroxidase.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3 (CD3 Produkte)

Hintergrund: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gen-ID: 12501

NCBI Accession: NP_031674

UniProt: P22646

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway