ZNF148 Antikörper

Produktnummer ABIN117932

Der Kaninchen Polyklonal Anti-ZNF148-Antikörper wurde für WB und EIA validiert. Er ist geeignet, ZNF148 in Proben von Human zu detektieren.

Kurzübersicht für ZNF148 Antikörper (ABIN117932)

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZNF148 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-ZNF148 Antikörper

Sequenz: 1 mniddklegl flkcggidem qssrtmvvmg gvsgqstvsg elqdsvlqdr smphqeilaa 61 devlqesemr qqdmishdel mvheetvknd eeqmetherl pqglqyalnv pisvkqeitf 121 tdvseqlmrd kkqirepvdl qkkkkrkqrs pakiltined gslglktpks hvcehcnaaf 181 rtnyhlqrhv fihtgekpfq csqcdmrfiq kyllqrheki htgekpfrcd ecgmrfiqky 241 hmerhkrths gekpyqceyc lqyfsrtdrv lkhkrmchen hdkklntcai kgglltseed 301 sgfstspkdn slpkkkrqkt ekkssgmdke saldksdlkk dkndylpvys sstkvkdeym 361 vaeyavemph ssvggshled asgeihppkl vlkkinskrs lkqpleqnqt isplstyees 421 kvskyafelv dkqalldseg nadidqvdnl qegpskpvhs stnyddamqf lkkkrylqaa 481 snnsreyaln vgtirsqpsv tqaavasvid esttasiles qalnveiksn hdknvipdev 541 lqtlldhysh kangqheisf svadtevtss isinssevpe vtpsenvgss sqasssdkan 601 mlqeyskflq qaldrtsqnd aylnspslnf vtdnqtlpnq pafssidkqv yatmpinsfr 661 sgmnsplrtt pdkshfgliv gdsqhsfpfs gdetnhasat stqdfldqvt sqkkaeaqpv 721 hqayqmssfe qpfrapyhgs ragiatqfst angqvnlrgp gtsaefsefp lvnvndnrag 781 mtsspdattg qtfg

Spezifität: This polyclonal antibody is specific for ZBP-89.

Aufreinigung: Delipidation and defibrination

Immunogen: Purified full length ZBP-89 recombinant protein expressed in E.coli

Anwendungsinformationen

Applikationshinweise: This polyclonal antibody reacts human ZBP-89 in a variety of immunological assaysincluding western blot and ELISA. This antibody is likely functional inimmunohistochemistry and immunoprecipitation. Recommended dilutionsImmunoblotting: 1: 5,000. A band at approximately 89 kDa corresponding to human ZBP-89is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937cells can be used as a positive control. ELISA: 1: 10,000 - 1: 30,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 90 mg/mL (by Refractometry)

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu ZNF148

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Andere Bezeichnung: ZNF148

Hintergrund: The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The fulllength protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.Synonyms: Transcription factor ZBP-89, ZBP89, Zinc finger DNA-binding protein 89, Zinc finger protein 148

Gen-ID: 7707

NCBI Accession: NP_068799

UniProt: Q9UQR1