ZNF148 Antikörper

Produktnummer ABIN964672

Produktdetails anti-ZNF148 Antikörper

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZNF148 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Spezifität: This product was prepared from monospecific antiserum by delipidation and defibrination. This polyclonal antibody is specific for human ZBP-89.  Reactivity with ZBP-89 from other species has not been determined.

Produktmerkmale: The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The full-length protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.

Sterilität: Sterile filtered

Immunogen: Purified full length ZBP-89 recombinant protein expressed in E.coli.
Immunogen Type: RecombinantProtein

Anwendungsinformationen

Applikationshinweise: This polyclonal antibody reacts human ZBP-89 in a variety of immunological assays including western blot and ELISA.  Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:5,000 dilution is recommended. A band at approximately 89 kDa corresponding to human ZBP-89 is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937 cells can be used as a positive control. For ELISA a 1:10,000 to 1:30,000 dilution is recommended. Researchers should determine optimal titers for other applications.

Kommentare:

Gene Name: ZBP-89

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 90 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-ZNF148 Antikörper (ABIN964672)

Ohneda, Ohmori, Ishijima, Nakano, Yamamoto: "Characterization of a functional ZBP-89 binding site that mediates Gata1 gene expression during hematopoietic development." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30187-99, (2009) (PubMed).

Guyot, Murai, Fujiwara, Valverde-Garduno, Hammett, Wells, Dear, Orkin, Porcher, Vyas: "Characterization of a megakaryocyte-specific enhancer of the key hemopoietic transcription factor GATA1." in: The Journal of biological chemistry, Vol. 281, Issue 19, pp. 13733-42, (2006) (PubMed).

Details zu ZNF148

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Andere Bezeichnung: ZBP-89 (ZNF148 Produkte)

Synonyme: BERF-1 antikoerper, BFCOL1 antikoerper, HT-BETA antikoerper, ZBP-89 antikoerper, ZFP148 antikoerper, pHZ-52 antikoerper, 2210405J08Rik antikoerper, AI480666 antikoerper, AW045217 antikoerper, Znf148 antikoerper, Zbp-89 antikoerper, fb06h05 antikoerper, wu:fb06h05 antikoerper, zfp149 antikoerper, zgc:165342 antikoerper, SLC12A8 antikoerper, zinc finger protein 148 antikoerper, ZNF148 antikoerper, Zfp148 antikoerper, znf148 antikoerper

Hintergrund: The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The full-length protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.
Synonyms: Transcription factor ZBP89 antibody, Zinc finger DNA binding protein 89 antibody, Zinc finger protein 148 antibody, ZNF 148 antibody

Gen-ID: 256711

UniProt: Q9UQR1