IL-8 Antikörper

Produktnummer ABIN1177284

Zitiert in 1 Publikation. Der Maus Monoklonal anti-IL-8 Antikörper (Klon 2A2) (ABIN1177284) detektiert spezifisch IL-8 in WB, ELISA und Neut. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für IL-8 Antikörper (ABIN1177284)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Klon: 2A2

Produktdetails anti-IL-8 Antikörper

Produktmerkmale: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-8. Neutralization activity may be measured with a calcium flux assay using 250 ng/mL recombinant human IL-8 (Cat. No. 554609) to stimulate calcium mobilization in 1x10^7 cells/mL human lysed whole blood as indicator cells. Preincubation of the antibody with recombinant human IL-8 can neutralize calcium mobilization.
50% Neutralization (ND50) at 8 - 10 myg/mL
>95% Neutralization at 150 - 500 myg/mL

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Recombinant human IL-8

Isotyp: IgG1 kappa

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-IL-8 Antikörper (ABIN1177284)

Matsushima, Oppenheim: "Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF." in: Cytokine, Vol. 1, Issue 1, pp. 2-13, (1991) (PubMed).

Details zu IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Andere Bezeichnung: IL-8

Hintergrund: The 2A2 antibody reacts with both the 72 and 77 amino acid forms of human IL-8. The immunogen used to produce the 2A2 hybridoma was E. coli-expressed recombinant human IL-8 (77 amino acid form). This antibody has been reported to show no cross-reactivity with the following cytokines or chemokines: Human IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-12 (p40 or p70), IL-13, IL-15, eotaxin, GRO, IFN- gamma, MCP-1, MCP-2, MCP-3, MIP-1alpha, MIP-1beta, NAP-2, PF4, RANTES, SCF, TNF, LT-alpha.

Pathways: TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagie