TBK1 Antikörper (pSer172) (Alexa Fluor 647)

Produktnummer ABIN1177215

Produktdetails anti-TBK1 Antikörper (Alexa Fluor 647)

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Bindungsspezifität: pSer172

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TBK1 Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Intracellular Staining (ICS)

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human TBK1

Klon: J133-587

Isotyp: IgG1 kappa

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Referenzen für anti-TBK1 Antikörper (Alexa Fluor 647) (ABIN1177215)

Viatour, Merville, Bours, Chariot: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." in: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, (2005) (PubMed).

Details zu TBK1

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Andere Bezeichnung: TBK1 (TBK1 Produkte)

Synonyme: nak antikoerper, t2k antikoerper, TBK1 antikoerper, wu:fk70c05 antikoerper, zgc:136548 antikoerper, NAK antikoerper, T2K antikoerper, 1200008B05Rik antikoerper, AI462036 antikoerper, AW048562 antikoerper, TANK binding kinase 1 L homeolog antikoerper, TANK binding kinase 1 antikoerper, TANK-binding kinase 1 antikoerper, tbk1.L antikoerper, TBK1 antikoerper, tbk1 antikoerper, Tbk1 antikoerper

Hintergrund: NF-kB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. In most cells, NF-kB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IkBalpha, IkBbeta, and IkBepsilon. Stimulation induces the release, activation, and nuclear translocation of NF-kB. Release of NF-kB results from the phosphorylation and proteolytic degradation of the IkB proteins. Two cytokine-inducible IkappaB kinases (IKKa and IKKb) phosphorylate and target the IkB proteins for degradation via the ubiquitin pathway. IKKg/NEMO, a third member of the IKK complex, functions as a regulatory subunit and interacts directly with IKKb. TBK1 (TANK-binding kinase 1, also known as T2K or NAK), a protein of 729 amino acids, is another member of the IKK family of kinases regulating NF-kB downstream of the tumor necrosis factor and Toll-like receptor pathways. TBK1 forms a complex with the adaptor proteins TANK (TRAF-associated NF-kB activator) and TRAF2 (TNF-receptor-associated factor 2), and this oligomer is required for activation and phosphorylation of TBK1 at serine 172 (S172). The J133-587 monoclonal antibody recognizes the phosphorylated S172 of human TBK1. Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7 because we have been unable to detect endogenous TBK1 (pS172) by western blotting of lysates from activated cell lines (HeLa activated with PMA and A431 activated with PDGF). In the transfectants, the over-expression of TBK1 is necessary for phosphorylation of IRF-7, but the presence of IRF-7 does not affect TBK1 expression or phosphorylation. We confirmed that mAb J133-587 does not cross-react with IRF-7 by ELISA.
Synonyms: T2K, NAK

Pathways: TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interaktom