Retinoblastoma 1 Antikörper (pSer780)

Produktnummer ABIN1177159

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Retinoblastoma 1 in ICS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für Retinoblastoma 1 Antikörper (pSer780) (ABIN1177159)

Target: Retinoblastoma 1 (RB1)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Retinoblastoma 1 Antikörper ist unkonjugiert

Applikation: Intracellular Staining (ICS)

Klon: J146-35

Produktdetails anti-Retinoblastoma 1 Antikörper

Bindungsspezifität: pSer780

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Rb Peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed by gel filtration chromatography. Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-Retinoblastoma 1 Antikörper (ABIN1177159)

Cobrinik: "Pocket proteins and cell cycle control." in: Oncogene, Vol. 24, Issue 17, pp. 2796-809, (2005) (PubMed).

Details zu Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Andere Bezeichnung: Rb

Hintergrund: The retinoblastoma gene product (Rb) is well known as a tumor suppressor and is either absent or mutated in many human tumors. Retrovirus-mediated gene transfer of the wild-type Rb gene into several Rb mutant neoplastic cell lines suppresses their tumorgenicity. Rb is a 110-kDa nuclear phosphoprotein that undergoes differential phosphorylation during the cell cycle. During G1 phase, Rb is predominantly in a hypophosphorylated state. It becomes increasingly phosphorylated throughout the cell cycle until late mitosis, when substantial dephosphorylation occurs. Hypophosphorylated Rb interacts with a number of cellular proteins including the E2F transcription factor, several cyclins, RBP-1, RBP-2, c-Abl, c-myc, N-myc, and p46. Phosphorylation of Rb at various sites, by Cyclin-dependent protein kinases, inhibits the binding of Rb to these proteins. Rb is thought to mediate its effects, in part, via the repression of genes required for proliferation. For example, Rb is specifically recruited to promoters containing E2F sites and actively represses E2F mediated transcription. Rb also stimulates the activity of other transcription factors, although the mechanisms are less clearly defined. Thus, Rb appears to regulate transcription in its aim to control cell growth. The J146-35 monoclonal antibody recognizes Rb phosphorylated at serine 780 (pS780), which affects Rb binding to E2F. The orthologous phosphorylation sites in mouse and rat Rb are serines 773 and 751, respectively.

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagie