PLK1 Antikörper (pThr210) (Alexa Fluor 647)

Produktnummer ABIN1177152

Zitiert in 1 Publikation. Der Alexa Fluor 647-konjugierte Maus Monoklonal anti-PLK1 Antikörper (Klon K50-483) (ABIN1177152) detektiert spezifisch PLK1 in ICS. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für PLK1 Antikörper (pThr210) (Alexa Fluor 647) (ABIN1177152)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PLK1 Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Intracellular Staining (ICS)

Klon: K50-483

Produktdetails anti-PLK1 Antikörper (Alexa Fluor 647)

Bindungsspezifität: pThr210

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human PLK1 Peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Referenzen für anti-PLK1 Antikörper (Alexa Fluor 647) (ABIN1177152)

Jang, Ma, Terada, Erikson: "Phosphorylation of threonine 210 and the role of serine 137 in the regulation of mammalian polo-like kinase." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44115-20, (2002) (PubMed).

Details zu PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Andere Bezeichnung: PLK1

Hintergrund: Polo-like kinase (PLK1) is a serine/threonine kinase with structural similarities to Drosophila's Polo kinase and the Cdc5p of Saccharomyces cerevisiae. Like its invertebrate counterparts, PLK1 activity is required for DNA synthesis and is regulated throughout the cell cycle. Furthermore, PLK1 is highly expressed in primary tumors. It associates with the mitotic spindle during mitosis suggesting that, in addition to its role during S phase, PLK1 may play a role during chromosome segregation. This is consistent with its potential role in cancer development. Threonine 210 (T210) is one of the major phoshorylation sites in activated PLK1 obtained from human mitotic cells. The K50-483 monoclonal antibody recognizes the phosphorylated T210 of human PLK1.

Pathways: Zellzyklus, M Phase