CD247 Antikörper (pTyr142) (Alexa Fluor 488)

Produktnummer ABIN1177046

Der Maus Monoklonal Anti-CD247-Antikörper wurde für ICS validiert. Er ist geeignet, CD247 in Proben von Human, Maus und Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für CD247 Antikörper (pTyr142) (Alexa Fluor 488) (ABIN1177046)

Target: CD247 (CD247 Molecule (CD247))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD247 Antikörper ist konjugiert mit Alexa Fluor 488

Applikation: Intracellular Staining (ICS)

Klon: K25-407-69

Produktdetails anti-CD247 Antikörper (Alexa Fluor 488)

Bindungsspezifität: pTyr142

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human CD3zeta Peptide

Isotyp: IgG2a kappa

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD247 Antikörper (Alexa Fluor 488) (ABIN1177046)

Alberola-Ila, Takaki, Kerner, Perlmutter: "Differential signaling by lymphocyte antigen receptors." in: Annual review of immunology, Vol. 15, pp. 125-54, (1997) (PubMed).

Details zu CD247

Target: CD247 (CD247 Molecule (CD247))

Andere Bezeichnung: CD247

Hintergrund: The T cell receptor (TCR), expressed by thymocytes and T lymphocytes, is a multi-component cell-surface complex responsible for recognizing antigen in the context of MHC molecules. The antigen-specific binding component of the TCR, Ti, is a heterodimer of the variable Ig-like subunits a and b or g and d. Ti is non-covalently associated with an invariant set of molecules referred to as the CD3 polypeptides, g, d, e, and zeta. The CD3 z polypeptide (CD3z) was named CD247 at the 7th Human Leukocyte Differentiation Antigens Workshop. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. After antigen recognition by the TCR, CD3z is the primary intracellular signal transducing subunit. It contains three ITAMs (Immunoreceptor Tyrosine-based Activation Motifs), each of which contains a pair of tyrosine residues that are phosphorylated by Lck and Fyn and are required for signal propagation. The molecular weight of CD3z is 16 kDa, and it is also observed as 32-kDa homodimers or as heterodimers with the g chain of Fc receptors. Upon phosphorylation, the CD3z monomer undergoes an apparent shift in electrophoretic mobility up to 21 kDa. The K25-407.69 monoclonal antibody recognizes the phosphorylated tyrosine 142 (pY142) in the third ITAM domain of human CD3z (CD247).
Synonyms: CD3zeta

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway