TBK1 Antikörper (C-Term) (PE)

Produktnummer ABIN1176941

Der Maus Monoklonal Anti-TBK1-Antikörper wurde für ICS validiert. Er ist geeignet, TBK1 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für TBK1 Antikörper (C-Term) (PE) (ABIN1176941)

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TBK1 Antikörper ist konjugiert mit PE

Applikation: Intracellular Staining (ICS)

Klon: 637Ig11-2

Produktdetails anti-TBK1 Antikörper (PE)

Bindungsspezifität: C-Term

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TBK1 C terminus Recombinant Protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-TBK1 Antikörper (PE) (ABIN1176941)

Viatour, Merville, Bours, Chariot: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." in: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, (2005) (PubMed).

Details zu TBK1

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Andere Bezeichnung: TBK1

Hintergrund: NF-kB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. In most cells, NF-kB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IkBalpha, IkBbeta, and IkBepsilon. Stimulation induces the release, activation, and nuclear translocation of NF-kB. Release of NF-kB results from the phosphorylation and proteolytic degradation of the IkB proteins. Two cytokine-inducible IkappaB kinases (IKKa and IKKb) phosphorylate and target the IkB proteins for degradation via the ubiquitin pathway. IKKg/NEMO, a third member of the IKK complex, functions as a regulatory subunit and interacts directly with IKKb. TBK1 (TANK-binding kinase 1, also known as T2K or NAK), a protein of 729 amino acids, is another member of the IKK family of kinases regulating NF-kB downstream of the tumor necrosis factor and Toll-like receptor pathways. TBK1 forms a complex with the adaptor proteins TANK (TRAF-associated NF-kB activator) and TRAF2 (TNF-receptor-associated factor 2), and this oligomer is required for activation and phosphorylation of TBK1 at serine 172. The 637Ig11.2 monoclonal antibody recognizes human TBK1, regardless of phosphorylation status. Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7 because we have been unable to detect endogenous TBK1 by western blotting of lysates from activated cell lines (HeLa activated with PMA and A431 activated with PDGF). In the transfectants, the over-expression of TBK1 is necessary for phosphorylation of IRF-7, but the presence of IRF-7 does not affect TBK1 expression or phosphorylation. We confirmed that mAb 637Ig11.2 does not cross-react with IRF-7 by western blot analysis using the purified antibody.
Synonyms: T2K, NAK

Pathways: TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interaktom