Myc Tag Antikörper

Produktnummer ABIN116942

Der Kaninchen Polyklonal anti-Myc Tag Antikörper wird verwendet zum Nachweis von Myc Tag. Er wurde validiert für WB, IHC (fro) und EIA.

Kurzübersicht für Myc Tag Antikörper (ABIN116942)

Target: Myc Tag

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Myc Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-Myc Tag Antikörper

Sequenz: EQKLISEEDL

Spezifität: Anti-Myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene. As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope. The antibody recognizes the Myc tag fused either to the amino- or carboxy- termini of targeted proteins. This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Aufreinigung: Affinity Chromatography.

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. Remarks: The sequence corresponds to aa 410-419 of Human c-Myc.

Anwendungsinformationen

Applikationshinweise: This antibody has been tested by ELISA (1/135,000) and Western blot (1/500-1/5,000)against both the immunizing peptide and myc containing recombinant proteins. Use1/400-1/2,000 for Immunohistochemistry on frozen sections. Although not tested, this antibody is likely functional for Immunoprecipitation andImmunocytochemistry.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative without stabilizers.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu Myc Tag

Target: Myc Tag

Andere Bezeichnung: C-Myc Epitope Tag

Substanzklasse: Tag

Hintergrund: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. We offer anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Please visit our website at www.acris-antibodies.de for more informations.Synonyms: c-myc tag, myc tag, myc-tag

Gen-ID: 4609

NCBI Accession: NP_002458

UniProt: P01106