PDPK1 Antikörper (AA 301-312)

Produktnummer ABIN116939

Der Kaninchen Polyklonal anti-PDPK1 Antikörper wird verwendet zum Nachweis von PDPK1 in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für PDPK1 Antikörper (AA 301-312) (ABIN116939)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDPK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PDPK1 Antikörper

Bindungsspezifität: AA 301-312

Sequenz: Peptide sequence: R-K-I-Q-E-V-W-R-Q-R-Y-Q-S

Spezifität: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is primarily directed against 60,000 MW human PDK-1 and is useful in determining its presence in various assays.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 301-312 of PDK-1 (genome data base accession number AC005591).

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody detects human PDK-1 and has been tested for use in ELISAand immunoblotting. Although untested, this reagent may be useful for Immunohistochemistry andImmunoprecipitation. It recognizes 60,000 MW human PDK-1. Reactivity in otherimmunoassays is unknown. Recommended Dilutions: This product has been assayed by immunoblot using HRPGoat-anti-Rabbit IgG [H&L] and Enhanced Chemiluminescence for detection. A workingdilution range of 1: 750 is suggested to detect PDK-1 in lysates derived from Sf9 insect cellsinfected with virus expressing myc-tagged human PDK1. In this experiment, approx. 10 μg of bothan untransfected sf9 cell lysate (not shown) and PDK-1 virus-infected Sf9 lysate wereseparated on a 10 % gel by SDS-PAGE followed by transfer to nitrocellulose. Primaryantibody reaction proceeded overnight at 4C using a 1: 750 dilution of the antibody in 1 %non-fat milk. This antibody predominantly recognizes a 60 kDa band corresponding tohuman PDK-1.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.3 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Andere Bezeichnung: PDPK1

Hintergrund: PDK-1 (3-Phosphoinositide-Dependant Protein Kinase-1) phosphorylates AGC kinases. PDK-1 activates conventional PKC and PKCz (zeta) through phosphorylation of critical threonine residues in the activation loop. PDK-1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase-3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.Synonyms: 3-phosphoinositide-dependent proteinkinase 1, PDK-1, PDK1, PDPK1, hPDK1

Gen-ID: 5170

NCBI Accession: NP_002604

UniProt: O15530

Pathways: PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals