MAVS Antikörper (AA 160-450)

Produktnummer ABIN1169132

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-MAVS Antikörper wird verwendet zum Nachweis von MAVS in Proben von Human. Er wurde validiert für WB, IHC, IP und ICC.

Kurzübersicht für MAVS Antikörper (AA 160-450) (ABIN1169132)

Target: MAVS (Mitochondrial Antiviral Signaling Protein (MAVS))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAVS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: Adri-1

Produktdetails anti-MAVS Antikörper

Bindungsspezifität: AA 160-450

Verwendungszweck: anti-Cardif (human), mAb (Adri-1)

Spezifität: Recognizes human Cardif.

Produktmerkmale: Monoclonal Antibody. Recognizes human Cardif. Isotype: Mouse IgG2b. Clone: Adri-1. Applications: ICC, IHC, IP, WB. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. RIG-I (retinoic acid-inducible gene I, Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-kappaB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-kappaB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-beta production.

Aufreinigung: Purified from concentrated hybridoma tissue culture supernatant.

Reinheit: >95 % (SDS-PAGE)

Immunogen: Recombinant human Cardif (aa 160-450).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-MAVS Antikörper (ABIN1169132)

Morikawa, Gouttenoire, Hernandez, Dao Thi, Tran, Lange, Dill, Heim, Donzé, Penin, Quadroni, Moradpour: "Quantitative proteomics identifies the membrane-associated peroxidase GPx8 as a cellular substrate of the hepatitis C virus NS3-4A protease." in: Hepatology (Baltimore, Md.), Vol. 59, Issue 2, pp. 423-33, (2014) (PubMed).

Bellecave, Sarasin-Filipowicz, Donzé, Kennel, Gouttenoire, Meylan, Terracciano, Tschopp, Sarrazin, Berg, Moradpour, Heim: "Cleavage of mitochondrial antiviral signaling protein in the liver of patients with chronic hepatitis C correlates with a reduced activation of the endogenous interferon system." in: Hepatology (Baltimore, Md.), Vol. 51, Issue 4, pp. 1127-36, (2010) (PubMed).

Michallet, Meylan, Ermolaeva, Vazquez, Rebsamen, Curran, Poeck, Bscheider, Hartmann, König, Kalinke, Pasparakis, Tschopp: "TRADD protein is an essential component of the RIG-like helicase antiviral pathway." in: Immunity, Vol. 28, Issue 5, pp. 651-61, (2008) (PubMed).

Details zu MAVS

Target: MAVS (Mitochondrial Antiviral Signaling Protein (MAVS))

Andere Bezeichnung: Cardif

Hintergrund:

Alternate Names/Synonyms: CARD Adapter Inducing Interferon-beta, IPS-1, MAVS, Mitochondrial Antiviral Signaling Protein, VISA, Virus-induced Signaling Adapter, Interferon-beta Promoter Stimulator Protein 1

Product Description: RIG-I (retinoic acid-inducible gene I, Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-kappaB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-kappaB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-beta production.

UniProt: Q7Z434

Pathways: Activation of Innate immune Response, Inositol Metabolic Process, Hepatitis C