PKM2 Antikörper

Produktnummer ABIN116809

Dieser Ziege Polyklonal Antikörper detektiert spezifisch PKM2 in WB, IP und EIA. Es zeigt Reaktivität gegenüber Proben von Kaninchen.

Kurzübersicht für PKM2 Antikörper (ABIN116809)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PKM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Produktdetails anti-PKM2 Antikörper

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Cross reactivity may occur against other mammalian Pyruvate Kinase, but were not specifically determined.

Aufreinigung: Prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: Pyruvate Kinase from Rabbit Muscle.

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation. Recommended Dilutions: This product has been assayed against 1.0 g of Pyruvate Kinase[Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as asubstrate for 30 minutes at room temperature. A working dilution of 1: 2,000 to 1: 12,000 ofthe reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 40.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Details zu PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Andere Bezeichnung: PKM2

Hintergrund: Synonyms: CTHBP, Cytosolic thyroid hormone-binding protein, M2-PK, OIP-3, OIP3, Opa-interacting protein 3, PK2, PK3, PKM, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle, Pyruvate kinase muscle isozyme, THBP1

Gen-ID: 100008676

NCBI Accession: NP_001182573

UniProt: P11974

Pathways: Warburg Effekt