Lactate Dehydrogenase A Antikörper

Produktnummer ABIN116756

Der Ziege Polyklonal Anti-Lactate Dehydrogenase A-Antikörper wurde für WB, IP, IF und EIA validiert. Er ist geeignet, Lactate Dehydrogenase A in Proben von Kaninchen zu detektieren.

Kurzübersicht für Lactate Dehydrogenase A Antikörper (ABIN116756)

Target: Lactate Dehydrogenase A (LDHA)

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Lactate Dehydrogenase A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-Lactate Dehydrogenase A Antikörper

Sequenz: Rabbit Lactate Dehydrogen ase A chain (muscle), 332 aa, predicted MW 36.6kDa 1 maalkdqlih nllkeehvpq nkitvvgvga vgmacaisil mkdladelal vdvmedklkg 61 emmdlqhgsl flrtpkivsg kdysvtansk lviitagarq qegesrlnlv qrnvnifkfi 121 ipnvvkysph ckllvvsnpv diltyvawki sgfpknrvig sgcnldsarf rylmgerlgv 181 halschgwil gehgdssvpv wsgmnvagvs lktlhpelgt dadkeqwkqv hkqvvdsaye 241 viklkgyttw aiglsvadla esimknlrrv hpistmlkgl ygikedvfls vpcvlgqngi 301 sdvvkvtlts eeeahlkksa dtlwgiqkel qf

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.

Aufreinigung: Prepared from monospecific antiserum by delipidation and defibrination

Immunogen: Full length lactate dehydrogenase protein isolated from Rabbit muscle.

Anwendungsinformationen

Applikationshinweise: Suitable for use in ELISA (1/4,000-1/20,000) and Western blot (1/500-1/2,000). Expect aband approximately 36 kDa in size corresponding to LDH western blotting in theappropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent)

Konzentration: 82 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide, None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Lactate Dehydrogenase A

Target: Lactate Dehydrogenase A (LDHA)

Andere Bezeichnung: LDHA

Hintergrund: Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.Synonyms: Cell proliferation-inducing gene 19 protein, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, NY-REN-59, PIG19, Renal carcinoma antigen NY-REN-59

Gen-ID: 100009107

NCBI Accession: NP_001075746

UniProt: P13491

Pathways: Warburg Effekt