Hexokinase 1 Antikörper

Produktnummer ABIN116752

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Hexokinase 1 in WB und EIA. Er zeigt eine Reaktivität gegenüber Saccharomyces cerevisiae.

Kurzübersicht für Hexokinase 1 Antikörper (ABIN116752)

Target: Hexokinase 1 (HK1)

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Hexokinase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Hexokinase 1 Antikörper

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Hexokinase [Yeast]. Cross reactivity against Hexokinase from other tissues and species may occur but have not been specifically determined.

Aufreinigung: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Hexokinase [Yeast].

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Hexokinase[Yeast] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 10,000 to1: 40,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 80.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Details zu Hexokinase 1

Target: Hexokinase 1 (HK1)

Andere Bezeichnung: Hexokinase-1

Hintergrund: Synonyms: Brain form hexokinase, HK1, Hexokinase type I

Gen-ID: 2542634

NCBI Accession: NP_592948

UniProt: Q09756

Pathways: Carbohydrate Homeostasis, Warburg Effekt