GFP Antikörper (Green Fluorescent Protein) (FITC)

Details for Product anti-GFP Antibody No. ABIN116747, Anbieter: Anmelden zum Anzeigen
Antigen
  • green fluorescent protein
  • gfp
Reaktivität
Aequorea victoria
637
18
5
5
2
2
1
1
Wirt
Ziege
336
176
79
43
16
9
9
2
Klonalität
Polyklonal
Konjugat
Dieser GFP Antikörper ist konjugiert mit FITC
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31
27
14
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10
9
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1
1
1
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1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
539
359
203
163
140
136
97
85
75
24
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Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
Spezifität Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
Produktmerkmale Absorption / Emission: 495 nm / 528 nm
Molar Ratio: 4.6 moles FITC per mole of Goat IgG
Reinigung Immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Andere Bezeichnung GFP
Substanzklasse Tag
Hintergrund Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
Gen-ID 6100
UniProt P42212
Applikationshinweise Polyclonal anti-GFP antibody is designed to detect GFP and its variants. This antibody canbe used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen andrecognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP antibody used in a sandwich ELISA is well suited to
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Restore with 1.0 mL of deionized water or equivalent.
Konzentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/mL BSA (IgG and Protease free) and 0.01 % (w/v) Sodium Azide as preservative
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing. Dilute only prior to immediate use.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below.
Produkt verwendet in: Niv, Keiner, Krishna, Witte, Lie, Redecker: "Aberrant neurogenesis after stroke: a retroviral cell labeling study." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2468-75, 2012 (PubMed).

Suzuki, Mogami, Ihara, Urano: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." in: Blood, Vol. 113, Issue 2, pp. 470-8, 2009 (PubMed).

Allgemeine Veröffentlichungen The, Feltkamp: "Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation." in: Immunology, Vol. 18, Issue 6, pp. 865-73, 1970 (PubMed).

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