Apolipoprotein C-II Antikörper

Produktnummer ABIN116545

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Apolipoprotein C-II in WB, IP, IHC (p), IHC (fro) und EIA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Apolipoprotein C-II Antikörper (ABIN116545)

Target: Apolipoprotein C-II (APOC2)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Apolipoprotein C-II Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-Apolipoprotein C-II Antikörper

Spezifität: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein C-II and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Aufreinigung: Immunoaffinity chromatography

Immunogen: ApoLipoprotein Type C-II produced synthetically in full-length form (not selected epitopes) using conventional peptide technology

Anwendungsinformationen

Applikationshinweise: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitationof antigen in serum using a standard curve, for Immunoprecipitation and for Westernblotting for highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by Immunoblot and found to be

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 with 0.01 % sodium azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Apolipoprotein C-II

Target: Apolipoprotein C-II (APOC2)

Andere Bezeichnung: Apolipoprotein C II (Apo CII)

Hintergrund: At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein C-II (ap °CII) is in found in chylomicrons (large lipoprotein particles absorbed from the gastrointestinal tract) and VLDL (large lipoproteins that are broken down to eventually form LDL). Ap °CII activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells. At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.Synonyms: APC2, Apo-CII, Ap °C-II, Ap °C2, Apolipoprotein C-II, Apolipoprotein C2

Gen-ID: 344

NCBI Accession: NP_000474

UniProt: P02655

Pathways: Lipid Metabolism